From da461b2e3ec4026f1e8805a7d3ac56ceb46e38f5 Mon Sep 17 00:00:00 2001
From: sanaxr0001-tech <sana.xr0001@gmail.com>
Date: Sat, 3 Oct 2026 12:51:05 +0100
Subject: [PATCH 1/4] Add NEST 96 well flat-bottom cell culture plate, 701011
 (nest_96_wellplate_200uL_Fb)

---
 .../img/nest/nest_96_wellplate_200uL_Fb.webp  | Bin 0 -> 29178 bytes
 docs/resources/library/nest.md                |   3 +-
 pylabrobot/resources/nest/plates.py           |  35 +++++++++++++
 pylabrobot/resources/nest/plates_tests.py     |  49 ++++++++++++++++++
 4 files changed, 86 insertions(+), 1 deletion(-)
 create mode 100644 docs/resources/library/img/nest/nest_96_wellplate_200uL_Fb.webp
 create mode 100644 pylabrobot/resources/nest/plates_tests.py

diff --git a/docs/resources/library/img/nest/nest_96_wellplate_200uL_Fb.webp b/docs/resources/library/img/nest/nest_96_wellplate_200uL_Fb.webp
new file mode 100644
index 0000000000000000000000000000000000000000..5e52cd007aec42aa0634d17e156f6342f8600ba4
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diff --git a/docs/resources/library/nest.md b/docs/resources/library/nest.md
index 19ee44fdf53..c8f69c866a0 100644
--- a/docs/resources/library/nest.md
+++ b/docs/resources/library/nest.md
@@ -10,4 +10,5 @@ Wuxi NEST Biotechnology Co., Ltd. a leading life science plastic consumables man
 | 'nest_1_troughplate_185000uL_Vb'<br>Part no.: 360101<br>[manufacturer website](https://www.nest-biotech.com/reagent-reserviors/59178415.html)<br>- Material: polypropylene | ![](img/nest/nest_1_troughplate_185000uL_Vb.webp) | `nest_1_troughplate_185000uL_Vb` |
 | 'nest_8_troughplate_22000uL_Vb'<br>Part no.: 360101<br>[manufacturer website](https://www.nestscientificusa.com/product/detail/513006470820794368)<br>- Material: polypropylene | ![](img/nest/nest_8_troughplate_22000uL_Vb.jpg) | `nest_8_troughplate_22000uL_Vb` |
 | 'nest_12_troughplate_15000uL_Vb'<br>Part no.: 360102<br>[manufacturer website](https://www.nestscientificusa.com/product/detail/513006470820794368)<br>- Material: polypropylene | ![](img/nest/nest_12_troughplate_15000uL_Vb.jpg) | `nest_12_troughplate_15000uL_Vb` |
-| 'NEST_96_wellplate_2200uL_Ub'<br>Part no.: 503062<br>[manufacturer website](https://www.nest-biotech.com/deep-well-plates/59253727.html)<br>- Material: polypropylene | ![](img/nest/NEST_96_wellplate_2200uL_Ub.png) | `NEST_96_wellplate_2200uL_Ub` |
\ No newline at end of file
+| 'NEST_96_wellplate_2200uL_Ub'<br>Part no.: 503062<br>[manufacturer website](https://www.nest-biotech.com/deep-well-plates/59253727.html)<br>- Material: polypropylene | ![](img/nest/NEST_96_wellplate_2200uL_Ub.png) | `NEST_96_wellplate_2200uL_Ub` |
+| 'nest_96_wellplate_200uL_Fb'<br>Part no.: 701011<br>[manufacturer website](https://www.nest-biotech.com/cell-culture-plates/59415537.html)<br>- Material: polystyrene<br>- Non-treated, sterile | ![](img/nest/nest_96_wellplate_200uL_Fb.webp) | `nest_96_wellplate_200uL_Fb` |
diff --git a/pylabrobot/resources/nest/plates.py b/pylabrobot/resources/nest/plates.py
index 7ae66387ff8..f5661529073 100644
--- a/pylabrobot/resources/nest/plates.py
+++ b/pylabrobot/resources/nest/plates.py
@@ -225,3 +225,38 @@ def NEST_96_wellplate_2200uL_Ub(name: str) -> Plate:
       name_prefix=name,
     ),
   )
+
+
+def nest_96_wellplate_200uL_Fb(name: str) -> Plate:
+  """NEST Cat. No. 701011: 96 well cell culture plate, flat bottom, non-treated, polystyrene.
+  Spec: NEST data sheet, "96 well cell culture plate, Flat bottom" technical drawing
+  http://www.gzjetway.com/upload/pdf/202506/04a71256d0b04cf888df963be987194b.pdf
+  Recommended medium volume: 0.1-0.2 mL, growth area: 0.32 cm2 per well
+  https://www.nest-biotech.com/cell-culture-plates/59415537.html
+  """
+  return Plate(
+    name=name,
+    size_x=127.56,  # from spec
+    size_y=85.36,  # from spec
+    size_z=14.30,  # from spec
+    lid=None,
+    model=nest_96_wellplate_200uL_Fb.__name__,
+    ordered_items=create_ordered_items_2d(
+      Well,
+      size_x=6.85,  # from spec (inner diameter at the rim)
+      size_y=6.85,  # from spec (inner diameter at the rim)
+      size_z=12.00,  # from spec (14.30 - 2.30)
+      dx=10.855,  # from spec (A1 center 14.28 - 6.85 / 2)
+      dy=7.755,  # from spec (row H center 11.18 from the front edge - 6.85 / 2)
+      dz=2.30,  # from spec (3.50 cavity floor - 1.20 floor thickness)
+      material_z_thickness=1.20,  # from spec
+      item_dx=9.0,  # from spec
+      item_dy=9.0,  # from spec
+      num_items_x=12,  # from spec
+      num_items_y=8,  # from spec
+      cross_section_type=CrossSectionType.CIRCLE,
+      bottom_type=WellBottomType.FLAT,
+      max_volume=200,  # from spec (upper end of the recommended medium volume, 0.1-0.2 mL)
+      name_prefix=name,
+    ),
+  )
diff --git a/pylabrobot/resources/nest/plates_tests.py b/pylabrobot/resources/nest/plates_tests.py
new file mode 100644
index 00000000000..f3a394f0546
--- /dev/null
+++ b/pylabrobot/resources/nest/plates_tests.py
@@ -0,0 +1,49 @@
+import unittest
+
+from pylabrobot.resources import Coordinate, Plate, nest_96_wellplate_200uL_Fb
+from pylabrobot.resources.well import CrossSectionType, WellBottomType
+
+
+class TestNest96Wellplate200uLFb(unittest.TestCase):
+  def setUp(self):
+    self.plate = nest_96_wellplate_200uL_Fb(name="plate")
+    self.plate.location = Coordinate.zero()
+
+  def test_footprint(self):
+    self.assertEqual(self.plate.get_size_x(), 127.56)
+    self.assertEqual(self.plate.get_size_y(), 85.36)
+    self.assertEqual(self.plate.get_size_z(), 14.30)
+
+  def test_layout(self):
+    self.assertEqual(self.plate.num_items_x, 12)
+    self.assertEqual(self.plate.num_items_y, 8)
+    self.assertEqual(len(self.plate.get_all_items()), 96)
+
+  def test_well_centers(self):
+    # The data sheet places the center of A1 14.28 mm from the left and 11.18 mm from the back edge,
+    # at a pitch of 9.00 mm; H12 is 99.00 mm to the right of A1 and 63.00 mm in front of it.
+    a1 = self.plate.get_well("A1").get_absolute_location("c", "c", "b")
+    self.assertAlmostEqual(a1.x, 14.28)
+    self.assertAlmostEqual(a1.y, 85.36 - 11.18)
+    h12 = self.plate.get_well("H12").get_absolute_location("c", "c", "b")
+    self.assertAlmostEqual(h12.x, 14.28 + 99.00)
+    self.assertAlmostEqual(h12.y, 85.36 - 11.18 - 63.00)
+
+  def test_well_geometry(self):
+    well = self.plate.get_well("A1")
+    self.assertEqual(well.get_size_x(), 6.85)
+    self.assertEqual(well.get_size_y(), 6.85)
+    self.assertEqual(well.bottom_type, WellBottomType.FLAT)
+    self.assertEqual(well.cross_section_type, CrossSectionType.CIRCLE)
+    self.assertEqual(well.max_volume, 200)
+
+    # The cavity is 10.80 mm deep: the rim is at the plate height and the floor is 1.20 mm thick.
+    rim = well.get_absolute_location("c", "c", "t").z
+    cavity_bottom = well.get_absolute_location().z + well.material_z_thickness
+    self.assertAlmostEqual(rim, 14.30)
+    self.assertAlmostEqual(cavity_bottom, 3.50)
+    self.assertAlmostEqual(rim - cavity_bottom, 10.80)
+
+  def test_serialize_round_trip(self):
+    plate = nest_96_wellplate_200uL_Fb(name="another_plate")
+    self.assertEqual(Plate.deserialize(plate.serialize()), plate)

From 12068cf0c4e523dc095d193719fc7b21633056f4 Mon Sep 17 00:00:00 2001
From: sanaxr0001-tech <sana.xr0001@gmail.com>
Date: Sun, 4 Oct 2026 14:07:01 +0100
Subject: [PATCH 2/4] nest_96_wellplate_200uL_Fb: add height_volume_data

Liquid height over the cavity bottom (mm) -> volume (uL) for the tapered well, computed as a
truncated cone from the NEST data-sheet drawing (6.40 mm across the floor, 6.85 mm across the rim,
10.80 mm deep). Points every 1.0 mm stay within 0.1 uL of the cone formula; the last point is the
brim-full volume, 372.4 uL at 10.80 mm.
---
 pylabrobot/resources/nest/plates.py       | 20 ++++++++++++++++++++
 pylabrobot/resources/nest/plates_tests.py | 20 ++++++++++++++++++++
 2 files changed, 40 insertions(+)

diff --git a/pylabrobot/resources/nest/plates.py b/pylabrobot/resources/nest/plates.py
index f5661529073..4e03df35f3b 100644
--- a/pylabrobot/resources/nest/plates.py
+++ b/pylabrobot/resources/nest/plates.py
@@ -227,6 +227,25 @@ def NEST_96_wellplate_2200uL_Ub(name: str) -> Plate:
   )
 
 
+# Liquid height over the cavity bottom (mm) -> volume (uL), computed from spec: the well is a
+# truncated cone, 6.40 mm across the floor and 6.85 mm across the rim, 10.80 mm deep. The last
+# point is the brim-full volume.
+_nest_96_wellplate_200uL_Fb_height_volume_data = {
+  0.0: 0.0,
+  1.0: 32.4,
+  2.0: 65.2,
+  3.0: 98.4,
+  4.0: 132.1,
+  5.0: 166.1,
+  6.0: 200.7,
+  7.0: 235.6,
+  8.0: 271.0,
+  9.0: 306.8,
+  10.0: 343.1,
+  10.8: 372.4,
+}
+
+
 def nest_96_wellplate_200uL_Fb(name: str) -> Plate:
   """NEST Cat. No. 701011: 96 well cell culture plate, flat bottom, non-treated, polystyrene.
   Spec: NEST data sheet, "96 well cell culture plate, Flat bottom" technical drawing
@@ -257,6 +276,7 @@ def nest_96_wellplate_200uL_Fb(name: str) -> Plate:
       cross_section_type=CrossSectionType.CIRCLE,
       bottom_type=WellBottomType.FLAT,
       max_volume=200,  # from spec (upper end of the recommended medium volume, 0.1-0.2 mL)
+      height_volume_data=_nest_96_wellplate_200uL_Fb_height_volume_data,
       name_prefix=name,
     ),
   )
diff --git a/pylabrobot/resources/nest/plates_tests.py b/pylabrobot/resources/nest/plates_tests.py
index f3a394f0546..8d988bdecf7 100644
--- a/pylabrobot/resources/nest/plates_tests.py
+++ b/pylabrobot/resources/nest/plates_tests.py
@@ -1,3 +1,4 @@
+import math
 import unittest
 
 from pylabrobot.resources import Coordinate, Plate, nest_96_wellplate_200uL_Fb
@@ -44,6 +45,25 @@ def test_well_geometry(self):
     self.assertAlmostEqual(cavity_bottom, 3.50)
     self.assertAlmostEqual(rim - cavity_bottom, 10.80)
 
+  def test_height_volume_data(self):
+    well = self.plate.get_well("A1")
+    cavity_depth = well.get_size_z() - well.material_z_thickness
+    self.assertEqual(well.compute_volume_from_height(0.0), 0.0)
+    assert well.height_volume_data is not None
+    self.assertAlmostEqual(max(well.height_volume_data), cavity_depth)
+    self.assertAlmostEqual(well.compute_volume_from_height(cavity_depth), 372.4)
+
+    # The well is a truncated cone, 6.40 mm across the floor and 6.85 mm across the rim.
+    def cone_volume(h: float) -> float:
+      r_floor, r_rim = 6.40 / 2, 6.85 / 2
+      r = r_floor + (r_rim - r_floor) * h / cavity_depth
+      return math.pi * h * (r_floor**2 + r_floor * r + r**2) / 3
+
+    for i in range(109):
+      h = i / 10
+      self.assertAlmostEqual(well.compute_volume_from_height(h), cone_volume(h), delta=0.1)
+    self.assertAlmostEqual(well.compute_height_from_volume(200), 5.98, places=2)
+
   def test_serialize_round_trip(self):
     plate = nest_96_wellplate_200uL_Fb(name="another_plate")
     self.assertEqual(Plate.deserialize(plate.serialize()), plate)

From 90c2b9182396f8931653ce8ace0d47e6e04e47af Mon Sep 17 00:00:00 2001
From: sanaxr0001-tech <sana.xr0001@gmail.com>
Date: Mon, 5 Oct 2026 10:10:45 +0100
Subject: [PATCH 3/4] nest_96_wellplate_200uL_Fb: max_volume is the brim-full
 volume

max_volume is 372.4 uL, the brim-full volume computed from the data-sheet drawing and the last
height_volume_data point. height_volume_data keeps three points: 0.0 mm, 6.0 mm (200.7 uL, about
the nominal 200 uL) and 10.8 mm (the brim), since the values are computed, not measured.
---
 pylabrobot/resources/nest/plates.py       | 15 +++------------
 pylabrobot/resources/nest/plates_tests.py |  9 ++++-----
 2 files changed, 7 insertions(+), 17 deletions(-)

diff --git a/pylabrobot/resources/nest/plates.py b/pylabrobot/resources/nest/plates.py
index 4e03df35f3b..4c9d284eb61 100644
--- a/pylabrobot/resources/nest/plates.py
+++ b/pylabrobot/resources/nest/plates.py
@@ -228,20 +228,11 @@ def NEST_96_wellplate_2200uL_Ub(name: str) -> Plate:
 
 
 # Liquid height over the cavity bottom (mm) -> volume (uL), computed from spec: the well is a
-# truncated cone, 6.40 mm across the floor and 6.85 mm across the rim, 10.80 mm deep. The last
-# point is the brim-full volume.
+# truncated cone, 6.40 mm across the floor and 6.85 mm across the rim, 10.80 mm deep. The points
+# are at about the nominal volume (200 uL) and at the brim.
 _nest_96_wellplate_200uL_Fb_height_volume_data = {
   0.0: 0.0,
-  1.0: 32.4,
-  2.0: 65.2,
-  3.0: 98.4,
-  4.0: 132.1,
-  5.0: 166.1,
   6.0: 200.7,
-  7.0: 235.6,
-  8.0: 271.0,
-  9.0: 306.8,
-  10.0: 343.1,
   10.8: 372.4,
 }
 
@@ -275,7 +266,7 @@ def nest_96_wellplate_200uL_Fb(name: str) -> Plate:
       num_items_y=8,  # from spec
       cross_section_type=CrossSectionType.CIRCLE,
       bottom_type=WellBottomType.FLAT,
-      max_volume=200,  # from spec (upper end of the recommended medium volume, 0.1-0.2 mL)
+      max_volume=372.4,  # computed from spec (brim-full, the last height_volume_data point)
       height_volume_data=_nest_96_wellplate_200uL_Fb_height_volume_data,
       name_prefix=name,
     ),
diff --git a/pylabrobot/resources/nest/plates_tests.py b/pylabrobot/resources/nest/plates_tests.py
index 8d988bdecf7..5cbbb5de932 100644
--- a/pylabrobot/resources/nest/plates_tests.py
+++ b/pylabrobot/resources/nest/plates_tests.py
@@ -36,7 +36,7 @@ def test_well_geometry(self):
     self.assertEqual(well.get_size_y(), 6.85)
     self.assertEqual(well.bottom_type, WellBottomType.FLAT)
     self.assertEqual(well.cross_section_type, CrossSectionType.CIRCLE)
-    self.assertEqual(well.max_volume, 200)
+    self.assertEqual(well.max_volume, 372.4)
 
     # The cavity is 10.80 mm deep: the rim is at the plate height and the floor is 1.20 mm thick.
     rim = well.get_absolute_location("c", "c", "t").z
@@ -51,7 +51,7 @@ def test_height_volume_data(self):
     self.assertEqual(well.compute_volume_from_height(0.0), 0.0)
     assert well.height_volume_data is not None
     self.assertAlmostEqual(max(well.height_volume_data), cavity_depth)
-    self.assertAlmostEqual(well.compute_volume_from_height(cavity_depth), 372.4)
+    self.assertEqual(max(well.height_volume_data.values()), well.max_volume)
 
     # The well is a truncated cone, 6.40 mm across the floor and 6.85 mm across the rim.
     def cone_volume(h: float) -> float:
@@ -59,9 +59,8 @@ def cone_volume(h: float) -> float:
       r = r_floor + (r_rim - r_floor) * h / cavity_depth
       return math.pi * h * (r_floor**2 + r_floor * r + r**2) / 3
 
-    for i in range(109):
-      h = i / 10
-      self.assertAlmostEqual(well.compute_volume_from_height(h), cone_volume(h), delta=0.1)
+    for h, volume in well.height_volume_data.items():
+      self.assertAlmostEqual(volume, cone_volume(h), delta=0.05)
     self.assertAlmostEqual(well.compute_height_from_volume(200), 5.98, places=2)
 
   def test_serialize_round_trip(self):

From f24f61b8ad1d992085f4b9394062b7a9863a0321 Mon Sep 17 00:00:00 2001
From: Camillo Moschner <camillo.moschner@biocam.guide>
Date: Wed, 7 Oct 2026 11:03:10 +0100
Subject: [PATCH 4/4] nest_96_wellplate_200uL_Fb: drop the definition tests

The tests restated the definition's own numbers; a pure labware definition needs no such protection.

Co-Authored-By: Claude Opus 5.5 <noreply@anthropic.com>
---
 pylabrobot/resources/nest/plates_tests.py | 68 -----------------------
 1 file changed, 68 deletions(-)
 delete mode 100644 pylabrobot/resources/nest/plates_tests.py

diff --git a/pylabrobot/resources/nest/plates_tests.py b/pylabrobot/resources/nest/plates_tests.py
deleted file mode 100644
index 5cbbb5de932..00000000000
--- a/pylabrobot/resources/nest/plates_tests.py
+++ /dev/null
@@ -1,68 +0,0 @@
-import math
-import unittest
-
-from pylabrobot.resources import Coordinate, Plate, nest_96_wellplate_200uL_Fb
-from pylabrobot.resources.well import CrossSectionType, WellBottomType
-
-
-class TestNest96Wellplate200uLFb(unittest.TestCase):
-  def setUp(self):
-    self.plate = nest_96_wellplate_200uL_Fb(name="plate")
-    self.plate.location = Coordinate.zero()
-
-  def test_footprint(self):
-    self.assertEqual(self.plate.get_size_x(), 127.56)
-    self.assertEqual(self.plate.get_size_y(), 85.36)
-    self.assertEqual(self.plate.get_size_z(), 14.30)
-
-  def test_layout(self):
-    self.assertEqual(self.plate.num_items_x, 12)
-    self.assertEqual(self.plate.num_items_y, 8)
-    self.assertEqual(len(self.plate.get_all_items()), 96)
-
-  def test_well_centers(self):
-    # The data sheet places the center of A1 14.28 mm from the left and 11.18 mm from the back edge,
-    # at a pitch of 9.00 mm; H12 is 99.00 mm to the right of A1 and 63.00 mm in front of it.
-    a1 = self.plate.get_well("A1").get_absolute_location("c", "c", "b")
-    self.assertAlmostEqual(a1.x, 14.28)
-    self.assertAlmostEqual(a1.y, 85.36 - 11.18)
-    h12 = self.plate.get_well("H12").get_absolute_location("c", "c", "b")
-    self.assertAlmostEqual(h12.x, 14.28 + 99.00)
-    self.assertAlmostEqual(h12.y, 85.36 - 11.18 - 63.00)
-
-  def test_well_geometry(self):
-    well = self.plate.get_well("A1")
-    self.assertEqual(well.get_size_x(), 6.85)
-    self.assertEqual(well.get_size_y(), 6.85)
-    self.assertEqual(well.bottom_type, WellBottomType.FLAT)
-    self.assertEqual(well.cross_section_type, CrossSectionType.CIRCLE)
-    self.assertEqual(well.max_volume, 372.4)
-
-    # The cavity is 10.80 mm deep: the rim is at the plate height and the floor is 1.20 mm thick.
-    rim = well.get_absolute_location("c", "c", "t").z
-    cavity_bottom = well.get_absolute_location().z + well.material_z_thickness
-    self.assertAlmostEqual(rim, 14.30)
-    self.assertAlmostEqual(cavity_bottom, 3.50)
-    self.assertAlmostEqual(rim - cavity_bottom, 10.80)
-
-  def test_height_volume_data(self):
-    well = self.plate.get_well("A1")
-    cavity_depth = well.get_size_z() - well.material_z_thickness
-    self.assertEqual(well.compute_volume_from_height(0.0), 0.0)
-    assert well.height_volume_data is not None
-    self.assertAlmostEqual(max(well.height_volume_data), cavity_depth)
-    self.assertEqual(max(well.height_volume_data.values()), well.max_volume)
-
-    # The well is a truncated cone, 6.40 mm across the floor and 6.85 mm across the rim.
-    def cone_volume(h: float) -> float:
-      r_floor, r_rim = 6.40 / 2, 6.85 / 2
-      r = r_floor + (r_rim - r_floor) * h / cavity_depth
-      return math.pi * h * (r_floor**2 + r_floor * r + r**2) / 3
-
-    for h, volume in well.height_volume_data.items():
-      self.assertAlmostEqual(volume, cone_volume(h), delta=0.05)
-    self.assertAlmostEqual(well.compute_height_from_volume(200), 5.98, places=2)
-
-  def test_serialize_round_trip(self):
-    plate = nest_96_wellplate_200uL_Fb(name="another_plate")
-    self.assertEqual(Plate.deserialize(plate.serialize()), plate)